Reporter

Part:BBa_K1750000:Design

Designed by: Janelle San Juan   Group: iGEM15_ANU-Canberra   (2015-09-18)


loxP-flanked RFP upstream from NPTII


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 103
    Illegal BamHI site found at 851
    Illegal XhoI site found at 1667
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 517
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Incorporation of a terminator sequence downstream of RFP to reduce leaky read-through of NPTII (though leaky read-through still occurs with this construct).


Source

Multispecies

References